Table 4.

Calculated kinetic parameters of the modified ribozymes

Observed reaction rate kobs (min−1)
RH1	0.112	RH1-8-2	0.022	RH1-L10	0.108
RH1-12-2	0.042	RH1-8-3	0.013	RH1-L11	0.008
RH1-12-3	0.040	RH1-8-4	0.004	RZ6A	0.015
RH1-12-4	0.002	RH1-8-5	0.003	RZ6A-12-2	0.003
RH1-12-5	0.057	RH1-8-6	n/n	RZ6A-12-8	0.008
RH1-12-6	0.026	RH1-8-7	0.002	RZ6C	0.128
RH1-12-7	0.002	RH1-8-8	n/n	RZ6C-12-2	0.020
RH1-12-8	0.077	RH1-8-9	0.029	RZ6C-12-8	0.093
RH1-12-9	0.032