TABLE 6.
Activities of enzymes involved in the EMP pathway and the pathway leading to the biosynthesis of sugar nucleotides and EPS in cell extracts of S. thermophilus NRa
Enzyme | Avg activity (nmol · mg of cell protein−1 · min−1) ± SD |
---|---|
α-Phosphoglucomutase | 154 ± 72 |
β-Phosphoglucomutase | 0 ± 0 |
UDP-glucose pyrophosphorylase | 18 ± 16 |
UDP-galactose 4-epimerase | 52 ± 28 |
dTDP-glucose pyrophosphorylase | 0 ± 1 |
Dehydratase | 7 ± 2 |
Epimerase reductase | 0 ± 0 |
Phosphoglucose isomerase | 1,687 ± 56 |
6-Phosphofructokinase | 2,064 ± 68 |
Fructose 1,6-bisphosphatase | 0 ± 0 |
S. thermophilus NR, a nonropy strain, was grown in batch cultures in MRS broth with 0.22 M lactose at 42°C and at a controlled pH 6.2. Samples were taken at the end of the exponential growth phase.