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. Author manuscript; available in PMC: 2023 Aug 1.

Published in final edited form as: Transplantation. 2021 Dec 23;106(8):1589–1599. doi: 10.1097/TP.0000000000004038

Figure 2. — A) Primary human RTECs were stimulated with media vs. necrotic supernatant for 30 minutes, washed twice and then lysed in 12 well plates. pMAPKs were then analyzed using Luminex. Y axis is luminescence standardized to the luminescence of beta actin, which served as a loading control. Graphs are representative of 3 separate experiments. Error bars represent SD of duplicates. *p<0.05, **p<0.01. B. RTECs were stimulated in same way as Fig 2A., analyzed for phospho- p38, JNK, and p53. Phosphotase treated lysates were used as a negative control. Data is representative of 3 separate experiments. Error bars are SDs of duplicates.

Figure 2. — A) Primary human RTECs were stimulated with media vs. necrotic supernatant for 30 minutes, washed twice and then lysed in 12 well plates. pMAPKs were then analyzed using Luminex. Y axis is luminescence standardized to the luminescence of beta actin, which served as a loading control. Graphs are representative of 3 separate experiments. Error bars represent SD of duplicates. *p<0.05, **p<0.01. B. RTECs were stimulated in same way as Fig 2A., analyzed for phospho- p38, JNK, and p53. Phosphotase treated lysates were used as a negative control. Data is representative of 3 separate experiments. Error bars are SDs of duplicates.