Scheme 2.

Characteristic fragment series generated by gas-phase activation of NA precursor ions. Typical a-B fragments are generated by the concomitant loss of nucleobase and cleavage of the 3’ C-O bond. B corresponds to any nucleobase. R corresponds to H in deoxyribo-ONs; OH in ribo-ONs; and CH3O in 2’O-methyl-ribo-ONs. X corresponds to OH in deoxyribo- and ribo-ONs; SH in phosphorothioate-ONs; and CH3 in methylphosphonate-ONs.