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. 2022 Jun 22;12:10552. doi: 10.1038/s41598-022-14104-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of secreted SUPYN protein in the TS21 maternal serum. (a) SUPYN-specific ELISA to monitor maternal SUPYN serum levels in normal pregnancies. (b) Quantification of secreted SUPYN protein (ng/ml) in serum from TS21 and gestational age-matched control pregnancies using a SUPYN-specific ELISA. Statistical analysis was performed using the Mann Whitney U test. Statistical significance was set at * : p < 0.05. Values represent mean ± SD (n > 5).