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. 2022 Jun 22;12:10552. doi: 10.1038/s41598-022-14104-1

Figure 3.

Figure 3

Association between SUPYN and ASCT2 induces glycosylation changes in TS21 placentas. (a) ASCT2 western blot analysis using gestational age-matched disomic control and TS21 placental samples. The numbers 1–4 represent sample replicates. Quantitative analysis of the percentage of ASCT2 in un-glycosylated bands when compared to total ASCT2 is presented at the bottom of the diagram. Glycosylated and un-glycosylated ASCT2 band intensities were quantified using CS analysis software from ATTO (2110030, Tokyo, JAPAN). The un-glycosylated ASCT2 ratio (%) represents the un-glycosylated band intensity / total band intensity × 100. (b) Average ratios combining all 4 replicates are graphically depicted. Statistical analysis was performed using the Mann Whitney U test. Statistical significance was set at * : p < 0.05. Values represent mean ± SD (n = 4).