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. 2022 Jun 22;12:10552. doi: 10.1038/s41598-022-14104-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SUPYN knock-down increases CGB gene expression in TS21 primary cells. SUPYN-specific mRNA knock-down in TS21 primary cells using a siRNA (siSUPYN) or control siRNA (siC). (a) Quantitative RT-PCR results of ERVH48-1 transcripts. (b) Secreted SUPYN protein in siRNA-treated and untreated cells. SUPYN protein levels in primary trophoblast cell culture supernatants were quantified by a SUPYN-specific ELISA (ng/ml). Quantification was normalized to untreated samples cultured for similar time periods (48 or 72H) (c) SUPYN knock-down induced an increase in CGB gene expression. Expression is normalized to untreated samples cultured for similar time periods (48 or 72H). (d) The number of nuclei in SUPYN-expressing cells was counted and fusion indices were calculated. Experiments were performed using four independent samples with 5 independent microscopic fields evaluated for each sample. Values represent mean ± SD (n = 4). Statistical significance was set at *p < 0.05 and **p < 0.01 compared to matched scrambled siRNA-treated samples using Mann–Whitney U-test. n.s. : not significant.