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. 2022 Jun 9;12:804287. doi: 10.3389/fonc.2022.804287

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Copyright © 2022 Chen, Hong, Zhou and Qin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CDH2 promotes the growth of THCA cells. (A) The mRNA expression of CDH2 in human normal thyroid cells HTori-3 and THCA cell lines (TPC-1, KTC-1 and BCPAP) by qPCR assay. (B) After transfection with siRNA, the expression levels of CDH2 were examined using qPCR assay. (C) The protein levels of CDH2 after transfection with siRNA were detected by western blot assay. (D) After knocking down CDH2 expression, the proliferation ability of THCA cells TPC-1 and KTC-1 was significantly decreased using CCK8 assay. (E) After knocking down CDH2 expression, the Ki67 expression was tested by Immunofluorescence assay. *P < 0.05; **P < 0.01; ***P < 0.001.