Table 2.
Primer information for cloning, subcellular localization and qPCR analysis.
| Primer name | Primer sequences (5′–3′) | Product length (bp) | Annealing temperature (°C) | Purpose | Efficiency |
|---|---|---|---|---|---|
| GSP | TTTTCCGCTTTTGTGGACGAA | 68 | 5′ RACE | ||
| NGSP | TGTGCTGGATTCCTCCCTGA | 68 | |||
| LXRα_F1 | GGTTTAAGAGTGGCCCTGACATCACA | 1601 | 61.6 | Gene cloning | |
| LXRα_R1 | CTTTAATGCCACGTGAGGATCTCCC | ||||
| LXRα_F2 | ctcgagATGTCTTTGTGGCTGGAGGC | 1344/1203/1077 | 60.1 | Subcellular localization | |
| LXRα_R2 | gaattcGTTCGTGCACATCCCAGATCT | ||||
| LXRα1 + 2_qF | CCAGAGGCCACAGAGCTTCGTC | 289 | 62.4 | LXRα1 + LXRα2 | 1.90 |
| LXRα1 + 2_qR | ATAAGACACACTCCTCCCGCAT | ||||
| LXRα2_qF | AGGCCACAGGTGTCTTATCAGAA | 225 | 60.1 | LXRα2 | 1.91 |
| LXRα2_qR | GCCAAGGCGTGACTCGAAGC | ||||
| LXRα3_qF | CCAGATCGCCCTGCTGAAGACG | 269 | 59.1 | LXRα3 | 1.87 |
| LXRα3_qR | AACATCAGTCGGTCCTGCTG | ||||
| ACTB_qF | TGGGCATGGAATCCTG | 196 | 60 | ACTB | 1.94 |
| ACTB_qR | GGCGCGATGATCTTGAT | ||||
| GAPDH_qF | ATGGAGAAGGCTGGGGCTCA | 144 | 60 | GAPDH | 1.92 |
| GAPDH_qR | GCAGGAGGCATTGCTGACAA | ||||
| RPS23_qF | ACCGACGAGACCAGAAGT | 306 | 60 | RPS23 | 1.91 |
| RPS23_qR | CTCCAGGAATGTCACCAA |
The lowercase letters are the restriction sites of XhoI and EcoRI.