Table 1.

Overview of knockout methods, genotypes, and cell lines created, as indicated by the 23 knocked out genes as well as AAVS1 control and WT cell lines.

Method	Description	Genes
	WT	WT (1)
Gene trap	KI/mut	AAVS1_Con (1), KMT2C (1), LPIN1 (1), NSUN3 (1), PLD3 (1), THUMPD3 (1), ZDHHC5 (1)
Gene trap	KI/KI	ACOT7 (1), ACSL3 (2), CEPT1 (1), CERS2 (1), DECR2 (1), DLAT (2), ELOVL5 (1), GNPAT (1), HSD17B12 (1), KMT2C (1), MPDU1 (1), NSUN2 (1), ORMDL2 (2), SAMD8 (1), SGMS1 (1), ZDHHC12 (2)
Deletion	KO/KO	ALDH3A2 (2), CEPT1 (2), CERS2 (2), FADS3 (1), GNPAT (1)

The number of clonal cell lines are indicated in parentheses.