Figure 5.

KOC-2S or SKOV3 cells were embedded in collagen gel droplets as described in Materials and Methods and cultured in 2 ml media containing 15 μM doxorubicin (DOX) with neutrophils (1 × 10⁷) and/or DNAse I (1000U/ml). NETs (-) and (+) show the data in the presence of unstimulated and LPS-stimulated neutrophils, respectively. After 12 h, apoptotic cells were examined with FACSCalibur. (A) Representative FACS Profiles of KOC-2S (B) Data are shown as mean ± standard deviation in triplicate from one of 4 (KOC-2S) and 3 (SCOV-3) different experiments. ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗: p < 0.001.