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. 2022 May 25;298(7):102077. doi: 10.1016/j.jbc.2022.102077

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

TXNRD3-deficient sperm undergo more oxidation after capacitation.A, free thiol groups level revealed with ThiolTracker in cauda epididymal sperm from wildtype and Txnrd3^−/− mice before and after 90 min capacitation. Arrowheads indicate the position of comparison, and empty arrowheads indicate absence/decrease of intensity. B, protein carbonylation level in sperm before and after capacitation, indicates a higher oxidation in capacitated Txnrd3^−/− sperm. The carbonyl groups derivatized by DNPH (2,4-dinitrophenylhydrazine) were detected and quantified by western blotting using DNP antibody.