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. 2022 May 25;298(7):102077. doi: 10.1016/j.jbc.2022.102077

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

TXNRD3-deficient sperm are more stable and resistant to acid denaturation after capacitation.A, sperm chromatin dispersion test evaluated smaller halo in Txnrd3^−/− sperm than that in wildtype sperm during capacitation, indicating extensive fragmented DNA after acid denaturation in Txnrd3^−/− sperm head. DNA was stained by DAPI (blue) after acid denaturation. B, quantification of halo sizes from wild type and Txnrd3^−/− sperm before and after capacitation. Based on sperm head size (∼8 μm), halo sizes are classified as small (<12 μm, ∼50% larger than head), medium (<17 μm, ∼100% larger), and big (>17 μm), respectively.