Fig. 6.

Alternative assembly routes observed in the reconstitution of type I collagen fibrils [17,36]. (A) Diagram to show three initiation routes to transfer the collagen from a cold acid solution to a warm neutral solution. Routes 1 and 2 involve a warming step and a neutralization step. Route 3 is a combined single step achieved by adding a warm buffer solution to the initial collagen solution. (B) Turbidimetric curves to show a comparison in the kinetics of fibril formation between the three routes. Both figures reprinted from Ref. [36] with a permission of publisher.