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Dot-blot assay of the selected aptamer: 15 aptamers were evaluated for binding affinity and specificity (A). Five aptamers named M12, M13, M15, M20, and M23 had higher affinity to mIL-2. The M15 and M20 aptamers bound to the target with high affinity compared to other aptamers, and neither aptamer nonreacted with His-tagged recombinant mouse TGF-β (B).
