Table 1.

The condition of 14 SELEX rounds.

Rounds	SELEX		Counter SELEX			Washing				Yeast tRNA	Desired PCR cycle
	mIL-2 (pmol)	Time∗∗	His-tag protein (pmol)	Naked Beads (μl)	Time∗∗	No.	Buffer (μl)	Time∗∗	Tween-20 0.05%
1	100	60	-	-	60	2	100	0.5	-	-	12
2	100	60	-	-		3	100	1	-	-	12
3	100	60	100	-		3	100	1	-	-	12
4	100	45	-	-		3	100	2	-	-	13
5	83	45	100	-		3	100	2	-	-	14
6	83	45	-	10		3	100	2	-	-	14
7	83	30	100	-		3	100	3	-	+	14
8	66	30	-	10		3	100	3	-	+	16
9	66	30	100	-		3	100	3	-	+	16
10	50	20	-	-		3	200	5	+	+	16
11∗	50	20	-	-		3	200	5	+	+	17
12	40	20	-	-		3	200	5	+	+	16
13	40	20	-	-		3	200	5	+	+	17
14	40	20	-	-		3	200	5	+	+	18

(∗Appropriate round, ∗∗ Minute).