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. 2022 Jun 15;54:102367. doi: 10.1016/j.redox.2022.102367

Fig. 4.

Fig. 4

DRP1-mediated mitochondrial fission triggers STING signaling activation in LPS-treated KCs. (A–B) Western blot analysis of DRP1 protein expression in KCs isolated from WT mice after Control or LPS treatment (n = 6). (C) Representative of immunofluorescence staining of MitoTracker and p-DRP1 in KCs (bar = 5 μm). (D–F) Mitochondrial network parameters including mitochondrial length, mitochondrial branch junctions and the percentage of mitochondrial with different lengths in KCs transfected with negative control vector (NC) or shDRP1 in the absence or presence of LPS (100 ng/mL for 12 h, n = 40 cells). (G) Co-location analysis of mitochondrial and p-DRP1 in each group (n = 12 cells). (H–K) Western blot analysis of STING, IRF3, p-IRF3, p65 and p-p65 protein expression in KCs transfected with NC or shDRP1 in the absence or presence of LPS (n = 4). Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.