Fig. 7.

The oxidative stress induced by DRP1-dependent mitochondrial fission enhances the release of mtDNA and subsequent STING signaling activation in LPS-treated KCs. (A–B) Representative of immunofluorescence staining of MitoSOX in KCs transfected with NC or shDRP1 in the absence or presence of LPS (n = 12 cells, bar = 5 μm). (C) Cytosolic mtDNA D-loops levels in KCs pre-treated with DMSO or MitoQ and then stimulated with LPS (n = 3). (D) IFN-β mRNA levels in KCs treated as in C (n = 3). Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.