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. 2022 Jun 15;54:102367. doi: 10.1016/j.redox.2022.102367

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 8 — Inhibition of mitochondrial fission with Mdivi-1 attenuates LPS-induced STING signaling activation in KCs and protects liver function. Primary hepatocytes and KCs were isolated from WT mice treated with Mdivi-1 20 mg/kg or Vehicle (equal volume of DMSO) in the absence or presence of LPS (10 mg/kg for 12 h). (A–E) Western blot analysis of STING, IRF3, p-IRF3, p65, p-p65, DRP1, p-DRP1 protein expression in KCs (n = 6). (F–H) Levels of inflammatory cytokines including IFN-β, TNF-α and IL-1β in mouse serum in each group (n = 4). (I–J) Hepatocyte death detection by flow cytometry in each group (n = 4). (K–L) H&E staining and histology scores of liver sections in each group (n = 6, bar = 100 μm). (M − N) ALT and AST concentrations in mouse serum in each group (n = 6). Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.

Fig. 8 — Inhibition of mitochondrial fission with Mdivi-1 attenuates LPS-induced STING signaling activation in KCs and protects liver function. Primary hepatocytes and KCs were isolated from WT mice treated with Mdivi-1 20 mg/kg or Vehicle (equal volume of DMSO) in the absence or presence of LPS (10 mg/kg for 12 h). (A–E) Western blot analysis of STING, IRF3, p-IRF3, p65, p-p65, DRP1, p-DRP1 protein expression in KCs (n = 6). (F–H) Levels of inflammatory cytokines including IFN-β, TNF-α and IL-1β in mouse serum in each group (n = 4). (I–J) Hepatocyte death detection by flow cytometry in each group (n = 4). (K–L) H&E staining and histology scores of liver sections in each group (n = 6, bar = 100 μm). (M − N) ALT and AST concentrations in mouse serum in each group (n = 6). Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.