Fig. 2.

Flow cytometry gating strategy to identify cytokine expressing CD4 and CD8 T cells. Doublets were excluded by forward scatter (FSC) area versus height gating. T cells were identified as CD3 positive and side scatter (SSC)-low cells and further subdivided into CD4 or CD8 positive cells (A). Interferon-gamma (IFN-γ), Interleukin-2 (IL-2), Interleukin-22 (IL-22) and tumor necrosis factor (TNF) expression in unstimulated (UNS) samples or after PPD and MIP stimulation in CD4 (B) and CD8 (C) T cells.