Fig. 6.

Histological, histochemical and protein analyses of the tumors treated with Mn-ZnO₂ NPs. (a) H&E-stained cross sections of the resected tumors with different treatments. Arrows of the insets indicate necrotic cells. (b) TUNEL staining (green) of the cross-sections of tumors with different treatments to detect the apoptotic cells. Nuclei were stained with DAPI (blue). (c) Semi-quantification of the TUNEL-positive areas shown in (b). Data are shown as mean ± SD. **p < 0.01. (d) Semi-quantification of the DHE fluorescence signal from different groups shown in (e). Fluorescence images (n ≥ 5 for each group) were analyzed by ImageJ (NIH). Data are shown as mean ± SD. **p < 0.01. (e) Fluorescence images of ROS generation within the tumors with different treatments. ROS was stained red with DHE staining. (f) Western blotting of Mutp53, ATM, WT p53, Bax, and GAPDH of the excised tumors with different treatments.