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. 2022 Jun 9;9:881630. doi: 10.3389/fmed.2022.881630

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Copyright © 2022 Rocha-Junior, Rodrigues, Slavov, Assone, Pedreschi, de La Roque, Sousa, Olavarria, Galvão-Castro, Fonseca, Penalva de Oliveira, Smid, Takayanagui, Casseb, Covas and Kashima.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amplification plots and standard curves of singleplex and multiplex assays. Singleplex format is demonstrated for (A,B) HTLV-1, (C,D) HTLV-2, and (E,F) internal control, beta-globin. (G,H) Multiplex format for the three targets HTLV-1, HTLV-2, and beta globin. Serial decimal dilutions ranging from 105 to 100 copies/reaction were used in the qPCR reactions. DNA from MT-2 and Gu cell lines were used as positive samples for HTLV-1 and HTLV-2, respectively.