Table 1.
Primers and probes sequences for HTLV-1, HTLV-2, and beta-globin (internal amplification control) detection.
| ID | Sequence (5′-3′) | Tm (°C) | GC (%) | Length (bp) |
|---|---|---|---|---|
| HTLV-1 For_pol | CAGCCCCTTCACAGTCTCTACTG | 59 | 57 | 23 |
| HTLV-1 Rev_pol | AGAAGGATTTAAATATATTTGGTCTCGG | 58.5 | 32 | 28 |
| HTLV-1 probe | FAM CCTTACAAAGGCATACTGAT MGB | 69 | 40 | 20 |
| HTLV-2 For_pol | CAAGGTGATGTAACCCATTATAAGTACAA | 58.8 | 34 | 29 |
| HTLV-2 Rev1_pol | AACCGCACCGGAGAAGGT | 59.1 | 61 | 18 |
| HTLV-2 Rev2_pol | AGAAACCAGCTGTGAGACTATCAGC | 59.1 | 48 | 25 |
| HTLV-2 probe | VIC AAATACAAATACTGCCTCCACGT MGB | 68 | 40 | 20 |
| Beta-globin For | TGAAGGCTCATGGCAAGAAA | 58 | 45 | 20 |
| Beta-globin Rev | GGTGAGCCAGGCCATCAC | 59 | 67 | 18 |
| Beta-globin probe | NED TGCTCGGTGCCTTT MGB | 69.0 | 63 | 14 |
ID, identification; Tm (°C), melting temperature in Celsius degrees; GC (%), percentage of G and C in the oligonucleotides sequences; bp, base pairs; MGB, minor groove binder. The length of amplicons is 75, 81, and 54 base pairs for HTLV-1, HTLV-2, and beta-globin, respectively.