Table 2.
Cycle threshold (Ct) values of repeatability (intra-assay) and reproducibility (inter-assay) analysis of duoplex-RT-PCR-HTLV 1/2 based on standard templates.
| Repeatability (intra-assay) * | ||||||
|---|---|---|---|---|---|---|
| HTLV-1 | HTLV-2 | |||||
| 105 copies/reaction | 104 copies/reaction | 103 copies/reaction | 105 copies/reaction | 104 copies/reaction | 103 copies/reaction | |
| μ ±σ | μ ±σ | μ ±σ | μ ±σ | μ ±σ | μ ±σ | |
| Run 1 | 22.88 ± 0.04 | 26.31 ± 0.12 | 29.82 ± 0.10 | 25.46 ± 0.16 | 28.92 ± 0.14 | 32.51 ± 0.22 |
| Run 2 | 22.57 ± 0.09 | 25.87 ± 0.07 | 29.41 ± 0.08 | 25.76 ± 0.16 | 29.09 ± 0.15 | 32.73 ± 0.11 |
| Run 3 | 22.57 ± 0.08 | 25.95 ± 0.08 | 29.68 ± 0.07 | 25.79 ± 0.16 | 29.22 ± 0.18 | 32.86 ± 0.14 |
| μ ±σ | 22.67 ± 0.16 | 26.04 ± 0.22 | 29.64 ± 0.19 | 25.67 ± 0.21 | 29.08 ± 0.20 | 32.70 ± 0.25 |
| %CV | 0.72 | 0.83 | 0.65 | 0.84 | 0.67 | 0.76 |
| Reproducibility (inter-assay) | ||||||
| Operator 1 | 22.44 ± 0.00 | 25.96 ± 0.01 | 29.66 ± 0.11 | 25.79 ± 0.01 | 28.87 ± 0.06 | 32.82 ± 0.07 |
| Operator 2 | 22.45 ± 0.02 | 25.92 ± 0.02 | 29.63 ± 0.26 | 25.73 ± 0.15 | 28.90 ± 0.06 | 32.98 ± 0.19 |
| Operator 3 | 22.49 ± 0.04 | 25.97 ± 0.03 | 29.91 ± 0.01 | 25.89 ± 0.10 | 29.23 ± 0.05 | 33.17 ± 0.14 |
| μ ±σ | 22.46 ± 0.03 | 25.95 ± 0.03 | 29.74 ± 0.19 | 25.80 ± 0.11 | 29.00 ± 0.18 | 32.99 ± 0.19 |
| % CV | 0.14 | 0.11 | 0.63 | 0.41 | 0.63 | 0.57 |
*
The repeatability assay was performed in five replicates of each standard dilution in three separated runs, using the same batch of reagents manipulated for a unique operator in the same day.