REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat, 1:5,000	Sigma-Aldrich	Cat# A8924, RRID: AB_258426
Monoclonal Anti-Langat Virus Envelope Glycoprotein (E), Clone 10F6, 3 :10,000	BEI Resources	NR-40316
Bacterial and virus strains
POWV strain LB	Ebel laboratory	Mandl et al., 1993
POWV-DTV strain Spooner	Ebel laboratory	Ebel et al., 1999
Experimental models: Cell lines
BHK-21 clone 13	ATCC	ATCC Cat# CCL-10, RRID: CVCL_1915
Other
Dulbecco’s Modified Eagle’s Media	Sigma-Aldrich	Cat# D5796-500ML
1 M HEPES solution	Sigma-Aldrich	Cat# H3537-100ML
Fetal bovine serum	Sigma-Aldrich	Cat# F0926
Methylcellulose	Sigma-Aldrich	Cat# M0512-250G
20% Electron microscopy grade paraformaldehyde	EMS	Cat# 15713-S
Saponin	Sigma-Aldrich	Cat# 47036
KPL TrueBlue Substrate	SeraCare	Cat# 5510-0052
ImmunoSpot® S6 FluoroCore M2 Analyzer	ImmunoSpot®	N/A