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Wash buffer
| Component (storage) | Stock | Final | 8 mL (for 4 samples) |
|---|---|---|---|
| Tris-HCl pH 7.4 (RT) | 1 M | 10 mM | 80 μL |
| NaCl (RT) | 5 M | 10 mM | 16 μL |
| MgCl2 (RT) | 1 M | 3 mM | 24 μL |
| BSA (4°C) | 10% | 1% | 800 μL |
| Tween-20 (RT) | 10% | 0.10% | 80 μL |
| Nuc Free water (RT) | – | 7 mL |
Store on wet ice or at 4°C (not more than 6 h) before use.
