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Sucrose cushion buffer I
| Component (storage) | Stock | Final | 12 mL (for 4 samples) |
|---|---|---|---|
| Nuclei PURE 2 M Sucrose Cushion Soln. (4°C; included in Nuclei Pure Prep Nuclei Isolation Kit) | – | – | 10.8 mL |
| Nuclei PURE Sucrose Cushion Buffer (4°C; included in Nuclei Pure Prep Nuclei Isolation Kit) | – | – | 1.2 mL |
Store on wet ice or at 4°C (not more than 6 h) before use.
