Diluted nuclei buffer
| Component (storage) | Stock | Final | 2 mL (for ∼ 4 samples) |
|---|---|---|---|
| Nuclei Buffer 20× (−20°C; included in Single Cell ATAC Library Kit) | 20× | 1× | 100 μL |
| Nuc Free water (RT) | 1900 μL |
Store on wet ice or at 4°C (not more than 1 h) before use.
Diluted nuclei buffer
| Component (storage) | Stock | Final | 2 mL (for ∼ 4 samples) |
|---|---|---|---|
| Nuclei Buffer 20× (−20°C; included in Single Cell ATAC Library Kit) | 20× | 1× | 100 μL |
| Nuc Free water (RT) | 1900 μL |
Store on wet ice or at 4°C (not more than 1 h) before use.