Figure 6.

$N{O}_2^{-}$ production by BMDMs is triggered by EVs of F. pedrosoi and F. nubica obtained from RM, but not from MM conditions. MM MC- stands for minimum medium without muriform cells and MM MC+ for minimum medium in the presence of muriform cells. Evs (80 ng/mL of sterol content) from F. pedrosoi (A), F. nubica (B), and F. erecta (C) grown in RM, MM MC-, and MM MC+ were incubated with 1x10⁶ BMDMs in the presence or absence of lipopolysaccharide (LPS, 500 ng/mL) for 24h following assessment of $N{O}_2^{-}$ production detected by Griess microplate assay. For all plots, * indicates p < 0.05, ** indicates p < 0.01, **** indicates p < 0.0001 compared to negative and positive control according to one-way analysis of variance (ANOVA). The results presented in this work refer to a representative triplicate group experiment of at least two independent assays.