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. 2022 Jun 6;26:15–26. doi: 10.1016/j.omto.2022.06.003

Figure 2.

Figure 2

Tandem incorporation of DAP10BBz fragment with GPC3-scFv confers T cell killing capacity against both GPC3+ and GPC3- cancer cells

(A) Schematic diagram of the structures of GPC3 CAR and GPC3-DAP10 CAR gene-expression cassettes. (B) Schematic diagram for the expression of GPC3-DAP10BBz with native NKG2D on T cell membrane. Arrows represent the interaction of NKG2D and DAP10. (C) Results of killing assays of CAR19-T, CAR GPC3-T, DAP10-T, and CAR GPC3-DAP10-T cells against Huh7-GL and SK-Hep1-GL cancer cell lines. The results shown are from one representative experiment out of two independent experiments with 2 different donors. (D) Detection of granzyme B secretion by T cells stimulated by Huh7-GL and SK-Hep1-GL cancer cell lines. (E) Detection of IFN-γ secretion by T cells stimulated by Huh7-GL and SK-Hep1-GL cancer cell lines. (F) Detection of IL-2 secretion by T cells stimulated by Huh7-GL and SK-Hep1-GL cancer cell lines. The killing results were compared with two-way ANOVA with Tukey’s multiple comparisons test. Student’s t test was used to compare cytokines secretion. Error bars denote the SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.