Figure 3.
Efficient elimination of GPC3 heterogeneous cancer cells by CAR GPC3-DAP10-T cells
(A) Schematic diagram of SK-Hep1 heterogeneous cancer cells. The GPC3+ (labeled by tdTomato) and GPC3- (labeled by EGFP) SK-Hep1 were mixed (ratio, 2:1) to mimic the heterogeneous cancer cells. The CAR-T cells (1 × 105) cocultured with heterogeneous SK-Hep1 (1 × 105) for 24 h in a 24-well plate, followed by imaging. (B) GPC3+ (red) and GPC3- (green) cancer cell counts after coculture with CAR19-T, DAP10-T, CAR GPC3-T, and CAR GPC3-DAP10-T cells. Seven individual photos on diagonal lines were used to calculate cell counts for one montage photo. (C) The overall plate images of indicated CAR-T cells. 8 × 8 photos for each CAR-T cells made one montage. The two yellow squares on montage photos indicate tow zoom-in photos below the montages. Scale bar, 200 μm. Error bars denote the SEM, and the results were compared with Student’s t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.