Figure 5.
CAR GPC3-DAP10-T cells can eliminate heterogeneous cancer in vivo
(A) Schematic diagram of the experiment. 5 × 105 GPC3+tdTomato+ SK-Hep1 cells and 5 × 105 GPC3-EGFP+ SK-Hep1 cells were mixed and transplanted into NSI mice. 5 × 106 CAR-T cells were injected via tail vein on day 0 when the tumor nodules were palpable. Mice were mercifully killed on day 33. (B) Measurement of SK-Hep1 tumor volumes after treatment with CAR19-T, DAP10-T, CAR GPC3-T, and CAR GPC3-DAP10-T cells. (C) Individual tumor responses to CAR-T cell injection are shown. (D) Tumor weights of the CAR19-T, DAP10-T, CAR GPC3-T, and CAR GPC3-DAP10-T cell groups on day 33 (time of sacrifice). (E) CAR-T cell infiltration in the tumor tissue harvested from mice. Tumor volumes were compared by two-way ANOVA with Tukey’s multiple comparisons test. Student’s t test was employed for tumor weights and CAR-T cell infiltration comparison. Error bars denote the SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.