TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source and/or referenceb |
|---|---|---|
| Strains | ||
| E. faecium | ||
| T136 | (Enterocins A and B) ADT indicator | OC (11) |
| P21 | (Enterocins A and B) | OC |
| P13 | (Enterocin P) ADT indicator | OC (11) |
| AA13 | (Enterocin P) | OC (22) |
| G16 | (Enterocin P) | OC (22) |
| L50 | (Enterocins L50A and L50B) | OC (15) |
| E. faecalis INIA 4 | (Enterocin AS-48) | INIA (30) |
| P. acidilactici | ||
| 347 | (Pediocin PA-1) | OC (40) |
| Ped− | P. acidilactici 347 isogenic strain (Non-pediocin PA-1 producer) | OC (33) |
| P. pentosaceus FBB61 | (Pediocin A) | TNO (37) |
| L. sakei | ||
| 706 | (Sakacin A) | NHL (23) |
| LTH673 | (Sakacin P) | NHL (48) |
| 148 | (Lactocin S) | OC (38) |
| L. lactis | ||
| BB24 | (Nisin A) | OC (39) |
| MG1614 | (Non-bacteriocin producer) | IFR (17) |
| IL1403 | Plasmid-free host strain | INRA (13) |
| Plasmids | ||
| pMC117 | Emr, pMG36e derivative carrying the pediocin operon under control of the lactococcal promoter P32 | 12 |
| pMG36c | Cmr, pMG36e derivative, gene expression vector | RuG-MG Laboratory collection |
| pHB04 | Cmr, pMG36c derivative carrying entA and orf2 under control of P32 | RuG-MG Laboratory collection |
| pJM03 | Cmr, pMG36c derivative carrying pedA and pedB under control of P32 | This work |
| pJM04 | Cmr, pHB04 derivative carrying pedA and pedB under control of P32 | This work |
a
The bacteriocin(s) produced is shown in parentheses. Em, erythromycin; Cm, chloramphenicol; r, resistance.
b
OC, our collection; IFR, Institute of Food Research, Norwich Laboratory (Norwich, United Kingdom); INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Madrid, Spain); INRA, Institut National de la Recherche Agronomique (Jouy-en-Josas, France); NHL, Laboratory of Microbial Gene Technology, Agricultural University (As, Norway); RuG-MG, Department of Molecular Genetics, University of Groningen, (Groningen, The Netherlands); TNO, Nutrition and Food Research (Zeist, The Netherlands).