TABLE 2.

Purification of enterocin A and pediocin PA-1 from L. lactis IL1403(pHB04) and P. acidilactici 347 supernatants, respectively

Supernatant and purification stage	Vol (ml)	Total A254a	Total activity (105 bacteriocin units)	Sp actb	Increase in sp actc (fold)	Yield (%)
L. lactis IL1403(pHB04)
Culture supernatant	1,000	30,500	15.8	52	1	100
Fraction
Ammonium sulfate precipitation	100	1,910	11.6	606	12	73
Gel filtration chromatography	200	980	7.4	752	14	47
Cation-exchange chromatography	50	6.9	1.2	16,726	322	7
Hydrophobic-interaction chromatography	10	2.75	6.3	2.3 × 105	4,390	40
RP-HPLC	0.75	0.06	1.2	2.1 × 106	39,956	7
P. acidilactici 347
Culture supernatant	1,000	23,900	61.0	255	1	100
Fraction
Ammonium sulfate precipitation	100	1,260	49.2	3,904	15	81
Gel filtration chromatography	200	390	37.0	9,487	37	61
Cation-exchange chromatography	50	16.7	30.5	1.8 × 105	706	50
Hydrophobic-interaction chromatography	10	9.3	29.1	3.1 × 105	1,216	48
RP-HPLC	1.15	0.51	500.0	98.0 × 106	384,314	820

^aA₂₅₄ is the absorbance at 254 nm multiplied by the volume in milliliters. 

^bSpecific activity is the number of bacteriocin units divided by total A₂₅₄. 

^cIncrease in specific activity is specific activity of a fraction divided by the specific activity of the culture supernatant.