TABLE 3.
Reactivities of anti-PH4 and anti-PH5 serum polyclonal antibodies against culture supernatants of LAB as determined by NCI-ELISA
| Microorganism (bacteriocin) | Cross-reactivity (%)a
|
|
|---|---|---|
| Anti-PH4 | Anti-PH5 | |
| E. faecium T136 (enterocins A and B) | 100 | 100 |
| E. faecium P21 (enterocins A and B) | 78.8 | 76.4 |
| P. acidilactici 347 (pediocin PA-1) | 22.9 | NR |
| P. acidilactici Ped− (non-pediocin PA-1 producer) | NR | 1.1 |
| E. faecium P13 (enterocin P) | 8.2 | NR |
| E. faecium AA13 (enterocin P) | 9.5 | NR |
| E. faecium G16 (enterocin P) | 13.9 | 1.5 |
| L. sakei 706 (sakacin A) | 6.9 | NR |
| L. sakei LTH673 (sakacin P) | NR | NR |
| E. faecium L50 (enterocins L50A and L50B) | NR | NR |
| P. pentosaceus FBB61 (pediocin A) | NR | NR |
| E. faecalis INIA 4 (enterocin AS-48) | NR | NR |
| L. sakei 148 (lactocin S) | NR | NR |
| L. lactis BB24 (nisin A) | NR | NR |
| L. lactis MG1614 | NR | NR |
a
Cross-reactivity is calculated as follows: [(absorbance reading produced by a culture supernatant above the absorbance reading produced by MRS/absorbance reading produced by the supernatant of E. faecium T136 above the absorbance reading produced by MRS) × 100]. NR, no reaction.