TABLE 4.
Pediocin PA-1 and enterocin A concentrations in culture supernatants as determined by NCI-ELISA with anti-PH2 and anti-PH5 antibodies
| Microorganism (bacteriocin) | Concn in supernatant with:
|
|||
|---|---|---|---|---|
| Anti-PH2
|
Anti-PH5
|
|||
| Pediocin PA-1 (ng/ml) | Cross-reactivitya (%) | Enterocin A (ng/ml) | Cross-reactivityb (%) | |
| E. faecium T136 (enterocin A and enterocin B) | NRc | 2,483 | 100 | |
| E. faecium P21 (enterocin A and enterocin B) | NR | 1,896 | 76.4 | |
| P. acidilactici 347 (pediocin PA-1) | 1,724 | 100 | NR | |
| L. lactis IL1403(pHB04) (enterocin A) | NR | 187 | 7.5 | |
| L. lactis IL1403(pJM03) (pediocin PA-1) | 115 | 6.7 | NR | |
| L. lactis IL1403(pJM04) (pediocin PA-1 and enterocin A) | 87 | 5.1 | 93 | 3.8 |
| L. lactis IL1403 | NR | NR | ||
a
Cross-reactivity is calculated as follows: [(concentration of pediocin PA-1 in a culture supernatant/concentration of pediocin PA-1 in the supernatant of P. acidilactici 347) × 100].
b
Cross-reactivity is calculated as follows: [(concentration of enterocin A in a culture supernatant/concentration of enterocin A in the supernatant of E. faecium T136) × 100].
c
NR, no reaction.