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. 2022 Jun 9;13:903755. doi: 10.3389/fimmu.2022.903755

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Copyright © 2022 Paudyal, Mwangi, Rijal, Schwartz, Noble, Shaw, Sealy, Bonnet-Di Placido, Graham, Townsend, Hammond and Tchilian

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NK cell degranulation activity of pb27 and pb39 IgG subclasses. (A) Schematic of the assay setup (figure was created with Biorender.com) and flow cytometry gating strategy for NK cells defined as CD3^-CD8α⁺. (B) Histogram normalized to mode showing the degranulation by pb27 and pb39 subclasses complexed with HA (red) and IgG only (blue). Anti-Nipah virus G protein IgG1 mAb was used as a control. (C) Bar chart showing degranulation by IgG complex with HA (red) or IgG only (blue). The error bars represent the SEM, n=4. (D) Assessment of degranulation titer of immune and naïve sera. Error bar represents the SEM, n=6.