Table 2.
Kinetic constants with standard error and fit parameters for pb27 and pb39 IgG subclasses.
| mAb | Subclass (new nomenclat ure) | Subclass (old nomencla ture) | MN(ng/ml) | KD (M) | KD Error | Ka(1/Ms) | KaError | Kdis(1/S) | KdisError | X2 | R2 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| pb27 | IgG1 | IgG1 | 5 | 5.6x10-12 | 1.5x10-14 | 2.9x106 | 1.7x103 | 1.6x10-5 | 4.4x10-8 | 29.969 | 0.99 |
| IgG2a | IgG2a | 5 | 4.1x10-12 | 6.1x10-15 | 4.3x106 | 1.7x103 | 1.7x10-5 | 2.5x10-8 | 2.7416 | 0.99 | |
| IgG2b | IgG4a | 5 | 4.8x10-12 | 2.4x10-14 | 2.4x106 | 1.6x103 | 1.2x10-5 | 6.1x10-8 | 102.355 | 0.99 | |
| IgG2c | IgG6.1 | 5 | 5.1x10-12 | 1.0x10-14 | 3.6x106 | 1.7x103 | 1.8x10-5 | 3.9x10-8 | 19.3179 | 0.99 | |
| IgG3 | IgG3 | 10 | 5.7x10-12 | 1.2x10-14 | 2.9x106 | 1.3x103 | 1.7x10-5 | 3.5x10-8 | 18.5426 | 0.99 | |
| IgG4 | IgG6.2 | 5 | 4.6x10-12 | 1.1x10-14 | 3.5x106 | 1.6x103 | 1.6x10-5 | 4.1x10-8 | 13.1264 | 0.99 | |
| IgG5b | IgG5.1 | 40 | 5.7x10-12 | 3.5x10-14 | 2.8x106 | 3.3x103 | 1.6x10-5 | 9.8x10-8 | 52.5166 | 0.99 | |
| IgG5c | IgG5.2 | 5 | 5.0x10-12 | 1.4x10-14 | 3.2x106 | 1.8x103 | 1.6x10-5 | 4.5x10-8 | 18.9703 | 0.99 | |
| pb39 | IgG1 | IgG1 | 640 | 1.2x10-10 | 7.1x10-13 | 1.1x105 | 2.6x102 | 1.3x10-5 | 7.1x10-8 | 27.0065 | 0.99 |
| IgG2a | IgG2a | 320 | 1.4x10-10 | 1.8x10-12 | 1.2x105 | 1.1x103 | 1.8x10-5 | 1.6x10-7 | 17.4855 | 0.99 | |
| IgG2b | IgG4a | 640 | 1.1x10-9 | 4.9x10-11 | 2.5x104 | 1.1x103 | 2.7x10-5 | 1.5x10-7 | 15.1037 | 0.99 | |
| IgG2c | IgG6.1 | 640 | 1.0x10-10 | 4.0x10-13 | 1.8x105 | 2.9x102 | 1.9x10-5 | 7.0x10-8 | 27.9447 | 0.99 | |
| IgG3 | IgG3 | 640 | 3.4x10-10 | 2.2x10-12 | 1.3x105 | 8.0x102 | 4.6x10-5 | 1.1x10-7 | 10.2786 | 0.99 | |
| IgG4 | IgG6.2 | 1280 | 2.0x10-10 | 8.3x10-13 | 9.0x104 | 2.2x102 | 1.8x10-5 | 5.9x10-8 | 37.2911 | 0.99 | |
| IgG5b | IgG5.1 | 1280 | 1.2x10-10 | 1.6x10-12 | 8.8x104 | 5.1x102 | 1.0x10-5 | 1.3x10-7 | 64.9777 | 0.99 | |
| IgG5c | IgG5.2 | 640 | 3.2x10-11 | 2.3x10-13 | 3.8x105 | 4.2x102 | 1.2x10-5 | 8.7x10-8 | 13.1969 | 0.99 |
MN, Microneutralization; KD, Dissociation equilibrium constant (affinity); Ka, Association rate constant; Kdis, Dissociation rate constant; X2,goodness of curve fitting; R2,Coefficient of determination.