Figure 1.

The condition-dependent E. coli metabolome

(A) The 19 conditions used in this study were adapted from the proteome study (Schmidt et al., 2016): three glucose-fed E. coli strains (BW25113, NCM3722, MG1655), 10 alternative carbon sources, three stresses (low pH, high salt, and high temperature), and stationary phase (1 day and 3 days). See also Table S1.

(B) Adenylate energy charge in the 19 conditions ([ATP]+ 0.5^.[ADP])/([ATP]+[ADP]+[AMP]). Bars are means from n = 3 replicates and error bars show the SD.

(C) Relative concentration of 101 intracellular E. coli metabolites. Concentrations are shown as log2 fold changes normalized to the glucose condition with E. coli BW25113. Dots show the mean of n = 3 replicates. Amino acids are shown in orange and nucleotides in blue. See also Table S2. Note that if a particular metabolite is supplemented to the cultivation medium, it is not possible to differentiate between its intracellular and extracellular concentration. Therefore, amino acids and adenine are excluded from the rich medium condition. Succinate is excluded from the succinate condition. See Figure S1–S3.