Figure 6.
The CMP mucus does not discharge in the vagina
(A) Immunofluorescence images representative of n = 3 wild-type mice showing Tff3 (green) produced in the epithelium of the cervical canal and found in the cervical mucus plug (CMP). Arrowheads outline Tff3+ cells.
(B) Wide-field immunofluorescence images representative of n = 3 transgenic Tg222 mice acquired using Axio Scan and showing Muc5b (green) in both the CMP and vaginal fornix (VF), whereas the transgene product (red) of Tff3-reporter mice was found only in the CMP. DNA was counterstained with Hoechst 33258 dye (blue). Ut, Uterus.
