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. 2022 May 30;25(7):104482. doi: 10.1016/j.isci.2022.104482
REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains

E. coli O86:B7 P.G. Wang (Georgia State University) N/A
P. alcalifaciens O5 and P. alcalifaciens O19 Y. Knirel (ND Zelinsky Institute of Organic Chemistry, Moscow, Russia) N/A
K. pneumoniae O 1 and K. pneumoniae O4 C. Whitfield (University of Guelph) N/A
S. pneumoniae 14 and S. pneumoniae 19 M. Nahm (University of Alabama at Birmingham) N/A
One Shot™ BL21(DE3) Chemically Competent E. coli Thermo Fisher Cat#C600003
One Shot™ TOP10 Chemically Competent E. coli Thermo Fisher Cat#C404006

Chemicals, peptides, and recombinant proteins

Human galectin-7 protein This study Plasmid from Emory Cloning Center
Human galectin-3 protein This study Plasmid from Emory Cloning Center
Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester) Thermo Fisher Cat#A20006
D-Lactose monohydrate, ACS Certified Grade, Fisher Chemical Thermo Fisher Cat#64044-51-5
2-Mercaptoethanol Sigma Cat# 60-24-2
Propidium Iodide Thermo Fisher Cat#P3566
SYTOX™ Orange Nucleic Acid Stain Thermo Fisher Cat#S11368
Isopropyl-β-D-thiogalactopyranoside (IPTG) Thermo Fisher Cat#BP1755100
Ampicillin Sigma Cat#A9518
Lb Broth Powder Thermo Fisher Cat#501977020
Triton X-100 Sigma Cat#9036-19-5
Thiodigalactoside Sigma Cat#51555-87-4

Critical commercial assays

DCFDA / H2DCFDA - Cellular ROS Assay Kit Abcam Cat#ab113851

Recombinant DNA

Human galectin-7 expression plasmid This study Plasmid from Emory Cloning Center
Human galectin-3 expression plasmid This study Plasmid from Emory Cloning Center

Software and algorithms

Prism - GraphPad GraphPad by Dotmatics Prism 8.4.2
GenePix® Pro 7 Microarray Acquisition & Analysis Software Molecular Devices GenePix Pro 7
GlycoStation ToolsPro GlycoTechnica GlycoStation®ToolsPro Ver.3.0
FlowJo BD Biosciences Version 10

Other

CFG Glycan microarray National Center for Functional Glycomics (NCFG) Version 3
Microbial glycan microarray (MGM array) National Center for Functional Glycomics (NCFG) PMCID: PMC4158828