| Bacterial and virus strains |
|
|
E. coli O86:B7 |
P.G. Wang (Georgia State University) |
N/A |
|
P. alcalifaciens O5 and P. alcalifaciens O19 |
Y. Knirel (ND Zelinsky Institute of Organic Chemistry, Moscow, Russia) |
N/A |
|
K. pneumoniae O 1 and K. pneumoniae O4 |
C. Whitfield (University of Guelph) |
N/A |
|
S. pneumoniae 14 and S. pneumoniae 19 |
M. Nahm (University of Alabama at Birmingham) |
N/A |
| One Shot™ BL21(DE3) Chemically Competent E. coli
|
Thermo Fisher |
Cat#C600003 |
| One Shot™ TOP10 Chemically Competent E. coli
|
Thermo Fisher |
Cat#C404006 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Human galectin-7 protein |
This study |
Plasmid from Emory Cloning Center |
| Human galectin-3 protein |
This study |
Plasmid from Emory Cloning Center |
| Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester) |
Thermo Fisher |
Cat#A20006 |
| D-Lactose monohydrate, ACS Certified Grade, Fisher Chemical |
Thermo Fisher |
Cat#64044-51-5 |
| 2-Mercaptoethanol |
Sigma |
Cat# 60-24-2 |
| Propidium Iodide |
Thermo Fisher |
Cat#P3566 |
| SYTOX™ Orange Nucleic Acid Stain |
Thermo Fisher |
Cat#S11368 |
| Isopropyl-β-D-thiogalactopyranoside (IPTG) |
Thermo Fisher |
Cat#BP1755100 |
| Ampicillin |
Sigma |
Cat#A9518 |
| Lb Broth Powder |
Thermo Fisher |
Cat#501977020 |
| Triton X-100 |
Sigma |
Cat#9036-19-5 |
| Thiodigalactoside |
Sigma |
Cat#51555-87-4 |
|
| Critical commercial assays |
|
| DCFDA / H2DCFDA - Cellular ROS Assay Kit |
Abcam |
Cat#ab113851 |
|
| Recombinant DNA |
|
| Human galectin-7 expression plasmid |
This study |
Plasmid from Emory Cloning Center |
| Human galectin-3 expression plasmid |
This study |
Plasmid from Emory Cloning Center |
|
| Software and algorithms |
|
| Prism - GraphPad |
GraphPad by Dotmatics |
Prism 8.4.2 |
| GenePix® Pro 7 Microarray Acquisition & Analysis Software |
Molecular Devices |
GenePix Pro 7 |
| GlycoStation ToolsPro |
GlycoTechnica |
GlycoStation®ToolsPro Ver.3.0 |
| FlowJo |
BD Biosciences |
Version 10 |
|
| Other |
|
| CFG Glycan microarray |
National Center for Functional Glycomics (NCFG) |
Version 3 |
| Microbial glycan microarray (MGM array) |
National Center for Functional Glycomics (NCFG) |
PMCID: PMC4158828 |