REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli (BL21(DE3))	Merck	Cat#69450
E. coli (BL21(DE3))	Bio-Rad	Cat#156-3003
E. coli (JM109(DE3))	Promega	Cat#P9801
E. coli (BL21-CodonPlus (DE3)-RIL)	Agilent	Cat#230245
E. coli (DH5α)	Takara	Cat#9057
Chemicals, peptides, and recombinant proteins
Recombinant human core histone H2A	Machida et al. (2018)	N/A
Recombinant human core histone H2B	Machida et al. (2018)	N/A
Recombinant human core histone H3.1	This paper	N/A
Recombinant human core histone H4	Machida et al. (2018)	N/A
Recombinant human p300(BRPHΔAILZ)	Zhang et al. (2018)	N/A
Recombinant human p300(BRPHΔAILZ) R1137A	This paper	N/A
Recombinant human p300(BRPHΔAILZ) BPD mutant (K1456A/K1459A/K1461A/R1462A)	This paper	N/A
Thrombin	Cytiva	Cat#27084601
Enterokinase, light chain	New England Biolabs	Cat#P8070L
Pre-Scission protease	Zhang et al. (2018)	N/A
Glutaraldehyde 25% solution, practical grade	Electron Microscopy Sciences	Cat# 16220-P
SYBR Gold	Thermo Fisher Scientific	Cat#S11494
DSS-H12/D12	Creative Molecules Inc.	Cat#001S
Trypsin/Lys-C Mix, Mass Spec Grade	Promega	Cat#V5072
Deposited data
p300(BRPHΔAILZ)-NCP complex I	This paper	EMDB: EMD-32373 PDB: 7W9V
p300(BRPHΔAILZ)-NCP complex II	This paper	EMDB: EMD-32374
p300(BRPHΔAILZ)-NCP complex III	This paper	EMDB: EMD-32375
p300(BRPHΔAILZ)-NCP complex IV	This paper	EMDB: EMD-32376
Crystal structure of nucleosome	Vasudevan et al. (2010)	PDB: 3LZ0
Crystal structure of the p300 acetyltransferase catalytic core	Kaczmarska et al. (2017)	PDB: 5LKU
Crosslinking mass spectrometry data	This paper	jPOST: JPST001584 PXID: PXD033804
Mendeley Data	This paper	https://doi.org/10.17632/dgcnhyz779.1
Recombinant DNA
pET-15b-H2A	Machida et al. (2018)	N/A
pET-15b-H2B	Machida et al. (2018)	N/A
pET-15b-H3.1 containing the enterokinase cleavage site	This paper	N/A
pET-15b-H4	Machida et al. (2018)	N/A
pGEX-6P-1-p300(BRPHΔAILZ)	Zhang et al. (2018)	N/A
pGEX-6P-1-p300(BRPHΔAILZ) R1137A	This paper	N/A
pGEX-6P-1-p300(BRPHΔAILZ) BPD mutant (K1456A/K1459A/K1461A/R1462A)	This paper	N/A
pGEM-T Easy-145 bp Widom 601 DNA	Arimura et al. (2013)	N/A
Software and algorithms
SerialEM ver.3	Mastronarde (2005)	https://bio3d.colorado.edu/SerialEM/
Relion 3.1.2	Zivanov et al. (2018)	https://www3.mrc-lmb.cam.ac.uk/relion//index.php/Main_Page
MOTIONCOR2 1.4.0	Zheng et al. (2017)	https://emcore.ucsf.edu/ucsf-software
CTFFIND 4.1.14	Rohou and Grigorieff (2015)	http://grigoriefflab.janelia.org/ctf
UCSF ChimeraX-1.2.5	Goddard et al. (2018)	https://www.cgl.ucsf.edu/chimerax/
COOT 0.9.5	Emsley et al. (2010)	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Phenix 1.19.2	Liebschner et al. (2019)	http://www.phenix-online.org
ISOLDE 1.2.2	Croll (2018)	https://isolde.cimr.cam.ac.uk/what-isolde/
MolProbity 4.5.1	Williams et al. (2018)	http://molprobity.biochem.duke.edu
xQuest/xProphet 2.1.3	Leitner et al. (2014)	https://gitlab.ethz.ch/leitner_lab/xquest_xprophet
ImageJ 1.52a	Schneider et al. (2012)	https://imagej.nih.gov/ij/
Other
Ni-NTA agarose beads	QIAGEN	Cat#30250
Pierce Glutathione Agarose	Thermo Fisher Scientific	Cat#16101
Mono S HR 16/10 column	Cytiva	Cat#17050701
HiTrap Q HP anion exchange column	Cytiva	Cat#17115401
HiLoad 16/600 Superdex 75 pg column	Cytiva	Cat#28989333
HiLoad 16/600 Superdex 200 pg column	Cytiva	Cat#28989335
Superdex 30 Increase 3.2/300 column	Cytiva	Cat#29219758
PD-10 column	Cytiva	Cat#17085101
Pierce C18 Tips	Thermo Fisher Scientific	Cat# 87784
C18 NANO HPLC CAPILLARY COLUMN	Nikkyo Technos Co., Ltd.	Cat# NTCC-360/75-3-125
Amicon Ultra-4 centrifugal filter unit (30,000 MWCO)	Merck	Cat#UFC803096
Amicon Ultra-2 centrifugal filter unit (30,000 MWCO)	Merck	Cat#UFC203024
Peristaltic pump	ATTO Corporation	Cat#1221200
Model 491 Prep Cell	Bio-Rad	Cat#1702928
Q5 Site-Directed Mutagenesis Kit	New England Biolabs	Cat#E0554S
QuikChange Lightning Site-Directed Mutagenesis Kit	Agilent	Cat#210518
SW 41 Ti Swinging-Bucket Rotor	Beckman Coulter	Cat#331362
Quantifoil R1.2/1.3 200-mesh Cu	Quantifoil	Cat#M2955C-1
Vitrobot Mark IV	Thermo Fisher Scientific
Krios G3i microscope	Thermo Fisher Scientific
UltiMate 3000 UHPLC system	Thermo Fisher Scientific
Orbitrap Fusion Tribrid mass spectrometer	Thermo Fisher Scientific