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. 2022 Jun 7;25(7):104542. doi: 10.1016/j.isci.2022.104542

Figure 5.

Figure 5

Identification of compounds by compound screening with selective cytotoxicity against Caco-2 cells

(A) Cytotoxic activity of 1 μM SN-38 against Caco-2 cells. Assays were performed in n = 6 independent biological replicates (mean ± S.D.). Statistical significance was determined by Student’s t test. ∗p < 0.05.

(B) Z′-factor values of the overall primary screening using Caco-2 cells.

(C) Scatterplots of primary screening data at a single concentration of 2 μM in Caco-2 cells and selection of hit compounds exhibiting >40% cytotoxic inhibition activity determined by CellTiter-Glo reagents.

(D) Dose-dependent analysis of compounds with prominently selective cytotoxicity against Caco-2 cells using dispersed IECs from hiPSOs, PIOs, and Caco-2 cells. Assays were performed in n = 4 independent biological replicates (mean ± S.D.).