Skip to main content
. 2022 Feb 3;101(7):802–811. doi: 10.1177/00220345211070222

Figure 4.

Figure 4.

Resveratrol improved periodontal ligament stem cell (PDLSC) immunomodulation in vitro and in vivo in a DSS-induced experimental colitis mouse model. (A) PDLSCs induced annexinV+7AAD+ double-positive apoptotic CD3+ T cells in a PDLSC/T-cell direct coculture system in vitro. Resveratrol treatment further increased annexinV+7AAD+ double-positive apoptotic CD3+ T cells. (B) Schema showing PDLSC transplantation in dextran sulfate sodium (DSS)–induced experimental colitis mice. (C) Colitis mice showed significantly reduced body weight from 8 to 12 d after DSS induction. The PDLSC transplantation, resveratrol-treated PDLSC transplantation, and 0.1× resveratrol-treated PDLSC transplantation groups showed inhibition of body weight loss compared to the colitis group at 12 d after DSS induction (n = 6 per group). (D) Disease activity index (DAI) was significantly increased in colitis mice compared to C57BL6 mice at 12 d after DSS induction. PDLSC transplantation, resveratrol-treated PDLSC transplantation, and 0.1× resveratrol-treated PDLSC transplantation significantly reduced DAI score. (E, G) Hematoxylin and eosin staining showed the infiltration of inflammatory cells (white arrows) in colon with destruction of epithelial layer in colitis mice. PDLSC transplantation, resveratrol-treated PDLSC transplantation, and 0.1× resveratrol-treated PDLSC transplantation rescued disease phenotype in colon and reduced histological activity index (G). Scale bar: 25 µm. (F, H) Immunofluorescence staining showed CD4+IL17+ Th17 cell infiltration in colitis. PDLSC transplantation, resveratrol-treated PDLSC transplantation, and 0.1× resveratrol-treated PDLSC transplantation dramatically reduced Th17 infiltration in colon. Scale bar: 25 µm. (I) Flow cytometry analysis further confirmed that Th17 cell level was significantly elevated in colitis mice compared to C57BL6 mice after DSS induction. PDLSC transplantation, resveratrol-treated PDLSC transplantation, and 0.1× resveratrol-treated PDLSC transplantation reduced the levels of Th17 cells in colitis mice. (J) Activated T cells were capable of inducing significant PDLSC death in a PDLSC/T-cell direct coculture system. Resveratrol treatment protected PDLSC apoptosis. Error bars represent the standard deviation from the mean values. *P < 0.05. ***P < 0.005.