FIG. 3.

Cultivation-independent PCR-SSCP profiles of bacterial communities isolated from rhizospheres of M. sativa (lanes 2 to 7) and C. album (lanes 8 to 13). Results from three replicate field plots are shown for each treatment. The results for plants from noninoculated field plots are shown in lanes 2 to 4 and 8 to 10. The results for plants from S. meliloti-inoculated plots are shown in lanes 5 to 7 and 11 to 13. The results for SSCP species standards, consisting of single-stranded DNA products obtained from PCR-amplified 16S rRNA gene regions (including regions V4 and V5) (see Materials and Methods) of selected bacterial species (Pf, Pseudomonas fluorescens; Bs, Bacillus subtilis; Sm, Sinorhizobium meliloti; Ar, Agrobacterium radiobacter), are shown in lanes 1 and 14.