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. 2022 Feb 3;101(7):832–839. doi: 10.1177/00220345211070583

Figure 1.

Figure 1.

Lef1 and Sostdc1 expression in normal and Msx1−/− tooth morphogenesis. (A–F; A′–F′) Expression pattern of Lef1 and Sostdc1 in frontal sections through the molar tooth germs in the control (A–F) and Msx1−/− (A′–F′) groups. Representative time points for early tooth development were examined: the placode (embryonic day 12.5 [E12.5]), bud (E13.5), and cap (E14.5) stages. In each panel, a white horizontal line divides the upper jaw (maxilla, Mx) and the lower jaw (mandible, Mn); the left side is palatal/lingual and right side is buccal. White arrowhead marks the palatal/lingual mesenchyme; black arrowhead marks the distal end of the tooth germ; red dashed line marks the basal lamina between the dental epithelium and mesenchyme. Scale bars, 100 µm. n = 4 for panels A–C and A′–C′; n = 10 for panels D–F and D′–F′. (G) Real-time quantitative polymerase chain reaction of Lef1 and Sostdc1 in Mx and Mn molar tooth germs in the control and Msx1−/− groups at bud-to-cap transition (at E14.0). Results are expressed as fold change ± SD relative to control Mx. n = 3 for each group. Student t test. *P ≤ 0.05. ***P ≤ 0.001. ns, not significant.