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. 2022 Apr 7;14(2):245–270. doi: 10.1016/j.jcmgh.2022.04.002

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Knockdown of TM9SF4 depleted ER calcium content in colonic epithelial cells. (A and B) HCECs with Scr-shRNA or TM9SF4-shRNAs were bathed in Ca²⁺-free physiological saline. Shown are time courses and a data summary of cytosolic calcium change in response to (A) 10 μmol ATP or (B) 5 μmol ionomycin (n = 4–5). Change in cytosolic calcium concentration was monitored with the Fluo-4 AM indicator. (C and D) HT-29 cells were overexpressed with TM9SF4 or pcDNA plasmids as control. Shown are time courses and a data summary of cytosolic calcium change in response to (C) 10 μmol ATP or (D) 5 μmol ionomycin (n = 4). (E and F) BMDMs with Scr-shRNA or TM9SF4-shRNAs were bathed in Ca²⁺-free physiological saline. Shown are time courses and a data summary of cytosolic calcium change in response to (E) 10 μmol ATP or (F) 5 μmol ionomycin (n = 4). Means ± SEM. ∗P < .05, ∗∗P < .01. pcDNA, plasmid cloning DNA; SCR, scrambled control.