Figure 11.

TM9SF4 regulated cytosolic pH value and cell surface expression of 2 phagocytosis-related proteins. Cytosolic pH values of (A) HCECs and (B) BMDMs were measured with pHrodo Red Indicator by flow cytometry. Shown are representative flow cytometer experiments and a data summary. (C) Whole-cell lysates of WT or KO BMDMs were collected, and cell surface proteins were isolated using surface biotinylation methods. The total and surface expressions of CD36 and MerTK were measured. ATP1A1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were the loading control for cell membrane proteins and total cellular proteins, respectively (n = 3). Means ± SEM. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. max, maximum; SCR, scrambled control.