Table 1.
Acceptor-sensitization FRET-binding curve parameters
| Enzymatic state | Stabilizing ligand(s) | PLB-SERCA |
DWORF–SERCA |
||
|---|---|---|---|---|---|
| KD (AU) | FRETmax (%) | KD (AU) | FRETmax (%) | ||
| E2 | TG | 4.4 ± 0.2 | 26 ± 0.8 | 2.7 ± 0.1 | 28 ± 0.5 |
| Eapo | 3.3 ± 0.2 | 25 ± 0.9 | 2.5 ± 0.2 | 29 ± 1.1 | |
| E1-ATP | ATP | 1.8 ± 0.2∗ | 22 ± 0.3 | 2.5 ± 0.2 | 29 ± 1.4 |
| E1 | Ca2+ | 3.5 ± 0.2 | 26 ± 0.9 | 2.6 ± 0.1 | 29 ± 0.7 |
| E1 | Ca2+-AMPPCP | 3.6 ± 0.2 | 26 ± 0.8 | 2.6 ± 0.2 | 29 ± 1.6 |
| E1P | Ca2+-ADP-AlF4 | 3.0 ± 0.1 | 22 ± 0.9 | 2.1 ± 0.2 | 25 ± 0.8 |
| E2P | AlF4 | 3.4 ± 0.2 | 24 ± 0.9 | 2.2 ± 0.3 | 27 ± 1.0 |
| E2P | Vi | 3.5 ± 0.3 | 26 ± 0.8 | 2.7 ± 0.3 | 31 ± 2.1 |
Data are reported as mean ± SEM (n = 6). Differences were determined by one-way ANOVA with Dunn’s post hoc (∗p < 0.05, See Table S1 for complete statistical analysis).