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. 2022 May 20;298(7):102059. doi: 10.1016/j.jbc.2022.102059

Figure 1.

Figure 1

RORα expression is downregulated in cells that undergo EMT.A, protein levels of RORα were examined by immunoblotting in Snail- and Twist-expressing HMLE cells. B, protein levels of RORα were examined by immunoblot in MCF10A and HMLE cells treated with TGF-β (10 ng/ml). C, EMT marker proteins were examined in control and RORα-silenced MCF10A cells. D, phase contrast and immunofluorescence images of control and two knockdown MCF10A cell lines (shRORα-1 and shRORα-2) in 2D culture. Bar represents 10 μm. E, phase contrast and immunofluorescent images of control and RORα-silenced MCF10A cells in 3D culture. Bar represents 10 μm. F, quantification of invasive colony ratio of control and RORα-silenced MCF10A cells in 3D culture; n = 5. ∗∗p < 0.01. EMT, epithelial–mesenchymal transition; RORα, retinoid orphan nuclear receptor alpha; TGF-β, transforming growth factor-β.